Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds

Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout world. disease considered to be one major diseases soybean and bean crops in Brazil. pathogen S. sclerotiorum spread by seeds both form sclerotia dormant mycelium inside seeds. objective this work was evaluate relationship between different potentials performance these seeds, as well verify localization quantification inoculum inoculated Real-time PCR (qPCR), validating term potential. Soybean were with osmotic conditioning method based on exposure for periods 24 h, 48 72 96 h. Molecular analysis carried out means qPCR whole dissected integument, cotyledon embryonic axis. results showed that effects seed germination vigor progressive proportional increases potentials, since there more severe damage consequently emerged plants at highest potential (P96). found all parts evaluated even its lowest (P24), an increasing DNA concentration, integument obtained a greater amount than embryo, comparison.